Amino acid uptake and homeostasis in Trypanosoma brucei
PhD Student
is available
The unicellular parasite Trypanosoma brucei is the causative agent of sleeping sickness in humans and Nagana in cattle. Besides their impact on public health, these parasites are interesting model organisms in cell and molecular biology.
Like other unicellular parasites, T. brucei depend on the import of amino acids for their metabolism. Therefore, amino acid transporters represent suitable drug targets and potential drug delivery systems.
Research in our lab aims at characterizing amino acid transport systems by
• Evaluating their role for parasite growth, development and pathogenicity
• Analyzing their functional properties, e.g. by determining substrate selectivity and affinity
• Determining the role of posttranslational modifications, e.g. phosphorylation, or alternative splicing for transport function, protein turnover, or regulation
Recently, a number of transporters that were upregulated during starvation for amino acids were identified. Work proposed includes determining the biochemical properties of two of these transporters by using heterologous expression systems (Saccharomyces cerevisiae and/or Xenopus laevis oocytes, electrophysiology, transport assays), elucidating their subcellular localization (by using YFP fusion proteins or other tags), and determining the physiological role in the parasite in more detail (RNAi, knockout, qRT-PCR, metabolite analyses). Furthermore, a major aim will be to identify upstream regulators which are controlling the starvation response by using a genome-wide screen.
Please contact:
Prof. Dr. Doris Rentsch, Write an email
http://www.ips.unibe.ch/plantphys
